Abstract: Notwithstanding their widespread diffusion, stereoscopic media have important drawbacks in terms of viewers’ visual discomfort. Current assessment methods are mainly based on measures of objective parameters such as eye physiology or media characteristics. On the other hand, subjective methods only evaluate the personal experience related to the physiological symptoms. In this pilot study we developed and validated the Stereoscopic Discomfort Scale (SDS), a self-assessment tool for the subjective evaluation of physiological and psychological symptoms related to stereoscopic viewing. The results show evidence of internal consistency, unidimensionality and construct validity of the scale. Since SDS scores were also strongly correlated with facets of presence, we argue that the SDS could be a useful tool for the investigation of users’ experience related to stereoscopic media.